RAD与新山地明协同抑制大鼠小肠移植中宿主抗移植物及移植物抗宿主免疫反应的作用

Synergistic effects of RAD and Neoral in inhibition of host-vs.-graft and graft-vs.-host immune responses in rat small-bowel transplantation.

作者信息

Johnson Stéphane, Qi Shiji, Xu Dasheng, Jolicoeur Marc, Liu Dingyi, Barama Azemi, Busque Stéphane, Smeesters Christian, Daloze Pierre, Chen Huifang

机构信息

Laboratory of Experimental Surgery, Research Center of CHUM, Notre-Dame Hospital, University of Montreal, Quebec, Canada.

出版信息

Microsurgery. 2003;23(5):476-82. doi: 10.1002/micr.10167.

DOI:10.1002/micr.10167

PMID:14558006

Abstract

The combined effects of RAD and Neoral were tested in a rat orthotopic small-bowel transplantation model. Seven groups (n = 6) were involved in this study, and each one was included in three rejection models for the evaluation of host-vs.-graft disease (HVG) (LBN-F1 to LEW), graft-vs.-host disease (GVH) (LEW to LBN-F1), and combined HVG and GVH immune responses (BN to LEW). Both drugs were administered orally throughout the study. Low doses of RAD (1.0-2.5 mg/kg/day) combined with Neoral (2.0-5.0 mg/kg/day) produced strong synergistic effects in the prolongation of small-bowel graft survival in HVG (combination index, CI = 0.095, 0.1212), GVH (CI = 0.027, 0.020), and combined HVG and GVH immune responses (CI = 0.070, 0.301). The combination therapy of RAD and Neoral produces a strong synergistic effect toward the inhibition of HVG, GVH, and combined HVG and GVH immune responses in a rat small-bowel transplantation model.

摘要

在大鼠原位小肠移植模型中测试了RAD和新山地明（Neoral）的联合效应。本研究纳入七组（n = 6），每组均包含三种排斥模型，用于评估宿主抗移植物病（HVG）（从LBN - F1到LEW）、移植物抗宿主病（GVH）（从LEW到LBN - F1）以及联合的HVG和GVH免疫反应（从BN到LEW）。在整个研究过程中，两种药物均通过口服给药。低剂量的RAD（1.0 - 2.5毫克/千克/天）与新山地明（2.0 - 5.0毫克/千克/天）联合使用，在延长HVG（联合指数，CI = 0.095，0.1212）、GVH（CI = 0.027，0.020）以及联合的HVG和GVH免疫反应（CI = 0.070，0.301）中的小肠移植物存活时间方面产生了强烈的协同效应。RAD和新山地明的联合疗法在大鼠小肠移植模型中对抑制HVG、GVH以及联合的HVG和GVH免疫反应产生了强烈的协同效应。