Resende Vanessa Contato Lopes, Montero Edna Frasson de Souza, Leão Jovelino Quintino De Souza, Manna Mônica Cecília Bochetti, Koike Márcia Kiyomi, Pedrosa Marcelo Eduardo, Simões Manuel De Jesus
Disciplina de Técnica Operatória e Cirurgia Experimental, Departamento de Cirurgia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Microsurgery. 2003;23(5):526-9. doi: 10.1002/micr.10162.
The aim of this study was to evaluate the early morphological development and acute rejection process in fetal intestine allografts. Grafts from C57BL/6 fetal intestines were implanted in an avascular form in BALB/C recipients. A syngeneic group of animals was used to compare the evolution. The allogeneic recipients were distributed in 6 groups, according to the day of sacrifice (3rd, 4th, 5th, 6th, 7th, and 10th postoperational day (POD)) and the control group on the 2nd, 5th, and 7th POD. These grafts were stained with hematoxylin and eosin for histological evaluation, in agreement with the classification of Auber et al. (Chirurgie 123:122-130, 1998). Data showed a progressive development of the graft until POD 5. On POD 3 and 4, a top grade of development and an initial rejection were observed. From POD 5-7 and on POD 10, the acute rejection reaction was more important than the development process. The higher level of rejection was observed on POD 10, and it was similar to the 7th POD. Our results showed good graft development until POD 5. After that, the acute rejection response impeded analysis of the development process.
本研究的目的是评估胎儿小肠同种异体移植的早期形态发育和急性排斥反应过程。将C57BL/6胎儿小肠的移植物以无血管形式植入BALB/C受体。使用同基因动物组进行对照比较。根据处死日（术后第3、4、5、6、7和10天（POD））将同种异体受体分为6组，对照组为术后第2、5和7天。按照奥伯等人（《外科学》123:122 - 130，1998）的分类，这些移植物用苏木精和伊红染色进行组织学评估。数据显示移植物在术后第5天之前逐渐发育。在术后第3天和第4天，观察到发育程度较高且出现初始排斥反应。从术后第5 - 7天以及术后第10天，急性排斥反应比发育过程更为显著。在术后第10天观察到最高水平的排斥反应，且与第7天相似。我们的结果表明移植物在术后第5天之前发育良好。之后，急性排斥反应阻碍了对发育过程的分析。
