Luo Liang, Luo Ya-ling, Lai Wen-yan
Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Oct;23(10):1069-73.
To investigate the effects of Huobahuagen tablet on the expression of interleukin-3 (IL-3) receptor mRNA in bronchoalveolar lavage fluid (BALF) and infiltration of eosinophil (Eos) in the airway of asthmatic guinea pigs.
Thirty-two healthy guinea pigs were randomized into 4 equal groups, the control group, asthmatic group, dexamethasone therapy group and Huobahuagen tablet therapy group. Asthma was induced in the latter 3 groups which were challenged with the asthma-inducing agents and at the same time received treatments as indicated. BALF were collected subsequently from the guinea pigs for examining the total cell number and cell classification, and histopathologic examination of the lung tissue was performed. Semi-quantitative analysis with reverse transcriptional-polymerase chain reaction (RT-PCR) of IL-3 receptor mRNA in the BALF was performed.
Compared with the control and the asthmatic group, the number of Eos in the BALF of Huobahuagen tablet therapy group was significantly lower (P<0.05 and P<0.01, respectively). In spite of the presence of hyperemia and edema in the lung tissues of the Huobahuagen tablet therapy group, Eos infiltration was less severe than that in the asthmatic group. As found by RT-PCR, the quantity of IL-3 receptor mRNA in the BALF of the Huobahuagen tablet therapy group did not significantly differ from that in the dexamethasone therapy group, but was significantly higher than that in both the control and asthmatic group (P<0.01).
Huobahuagen tablet can significantly lower the number of Eos in the airway of asthmatic guinea pigs, a finding that may potentially serve as the elementary theoretical basis for clinical therapy of asthma.
探讨火把花根片对哮喘豚鼠支气管肺泡灌洗液（BALF）中白细胞介素-3（IL-3）受体mRNA表达及气道嗜酸性粒细胞（Eos）浸润的影响。
将32只健康豚鼠随机分为4组，即对照组、哮喘组、地塞米松治疗组和火把花根片治疗组。后3组诱发哮喘，用致喘剂激发，并同时给予相应治疗。随后收集豚鼠的BALF，检测其细胞总数及细胞分类，并对肺组织进行病理检查。采用逆转录聚合酶链反应（RT-PCR）对BALF中IL-3受体mRNA进行半定量分析。
与对照组和哮喘组相比，火把花根片治疗组BALF中Eos数量显著降低（分别P<0.05和P<0.01）。火把花根片治疗组肺组织虽有充血、水肿，但Eos浸润程度较哮喘组轻。RT-PCR结果显示，火把花根片治疗组BALF中IL-3受体mRNA量与地塞米松治疗组相比差异无统计学意义，但显著高于对照组和哮喘组（P<0.01）。
火把花根片可显著降低哮喘豚鼠气道内Eos数量，这一结果可能为哮喘临床治疗提供初步理论依据。
