无细胞遗传电路组装原理。
Principles of cell-free genetic circuit assembly.
作者信息
Noireaux Vincent, Bar-Ziv Roy, Libchaber Albert
机构信息
Center for Studies in Physics and Biology, The Rockefeller University, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12672-7. doi: 10.1073/pnas.2135496100. Epub 2003 Oct 14.
Abstract
Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regions of linear response for single stages, cascading to subsequent stages requires working in nonlinear regimes. Substantial time delays and dramatic decreases in output production are incurred with each additional stage because of a bottleneck at the translation machinery. Faster turnover of RNA message can relieve competition between genes and stabilize output against variations in input and parameters.
摘要
无细胞遗传电路元件是在转录-翻译提取物中构建的。我们设计了转录激活和抑制级联反应，其中每个阶段的蛋白质产物是驱动或阻断下一阶段所需的输入。尽管我们可以找到单个阶段的线性响应区域，但级联到后续阶段需要在非线性区域工作。由于翻译机制的瓶颈，每增加一个阶段都会产生大量的时间延迟和输出产量的显著下降。更快的RNA信息周转可以缓解基因之间的竞争，并稳定输出以抵抗输入和参数的变化。
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