Lee J-J, Nam C-E, Kook H, Maciejewski J P, Kim Y-K, Chung I-J, Park K-S, Lee I-K, Hwang T-J, Kim H-J
Blood and Marrow Transplant Program, Chonnam National University Medical School, Gwangju, Korea.
Bone Marrow Transplant. 2003 Nov;32(9):947-52. doi: 10.1038/sj.bmt.1704253.
We evaluated the genotypic origin of mesenchymal stem cells (MSC) following sex-mismatched allogeneic bone marrow transplantation (BMT), and investigated the telomere dynamics in MSC in normal individuals and patients after BMT. The study population consisted of 11 patients with hematologic disorders who showed complete chimerism after BMT. Telomere length was measured in MSC using Southern blotting analysis in eight patients and 18 healthy subjects as a control group. Following culture, MSC were identified by the expression of SH2 and SH4, and lack of CD14, CD34, and CD45. All MSC showed the recipient genotype, based on the results of fluorescent in situ hybridization analysis using X-chromosome satellite probes or microsatellite DNA polymorphism analysis. The mean telomere length in MSC from normal controls was 7.2+/-0.53 kb (range, 6.12-7.78), and progressive telomere shortening was seen with age. There was no significant difference in MSC telomere length between the BMT group and age-matched controls. This study confirmed that the MSC isolated from the recipients of allogeneic BMT did not have the donor genotype, despite complete chimerism. Moreover, MSC were demonstrated to show progressive loss of telomere length with age, but the telomeres in MSC were not affected by BMT.
我们评估了性别不匹配的异基因骨髓移植（BMT）后间充质干细胞（MSC）的基因型来源，并研究了正常个体和BMT后患者MSC中的端粒动态变化。研究人群包括11例血液系统疾病患者，他们在BMT后表现出完全嵌合。在8例患者和18名健康受试者（作为对照组）中，使用Southern印迹分析测量MSC中的端粒长度。培养后，通过SH2和SH4的表达以及缺乏CD14、CD34和CD45来鉴定MSC。基于使用X染色体卫星探针的荧光原位杂交分析或微卫星DNA多态性分析的结果，所有MSC均显示出受体基因型。正常对照组MSC中的平均端粒长度为7.2±0.53 kb（范围为6.12 - 7.78），并且随着年龄的增长端粒逐渐缩短。BMT组和年龄匹配的对照组之间MSC端粒长度没有显著差异。这项研究证实，尽管完全嵌合，但从异基因BMT受者中分离出的MSC没有供体基因型。此外，已证明MSC的端粒长度会随着年龄的增长而逐渐丧失，但BMT不会影响MSC中的端粒。
