Schaller B, Graf R, Wienhard K, Heiss W-D
Max-Planck-Institute for Neurological Research, Cologne, Germany.
Swiss Med Wkly. 2003 Jul 26;133(29-30):412-8. doi: 10.4414/smw.2003.10236.
Dural sinus occlusion is an infrequent but potentially devastating cause of stroke. The pathophysiological course of events underlying it is, as yet incompletely understood.
In a cat model, regional cerebral blood flow (CBF) was measured during control and 2, and 24 hours after superior sagittal sinus occlusion. Around 48 hours after superior sagittal sinus occlusion, experimental settings were terminated by perfusion fixation with 4% paraformaldehyde solution, and haematoxylin and eosin histology.
CBF was significantly reduced over the time-period of measurement (p < 0.05) covering about 45% of the brain in planes that were affected by occlusion. Histologically, in all cases signs of subacute venous infarction could be demonstrated.
Based on the newly-developed model of microsurgical ligation of the superior sagittal sinus in cats, we present for the first time an animal model for cerebral venous infarction that leads to a histologically proven subacute venous infarction with a good reproducibility. The further advantage of this model is the fact that it mimics the clinical situation as far as possible by its inter- and intra-individual variance of extension of the venous infarction and by the slow reduction of CBF over 24 hours. Sequential PET imaging is a favourable, non-invasive method to gain further insight into the pathophysiological characteristics of experimental cerebral venous infarction. Therefore, the new-developed cat-model as demonstrated in this study will be of great value for further and more detailed investigations of cerebral-venous infarctions, and for the experimental evaluation of therapeutic strategies.
硬脑膜窦闭塞是一种罕见但可能导致毁灭性后果的中风病因。其潜在的病理生理过程尚未完全明确。
在猫模型中，于上矢状窦闭塞前、闭塞后2小时和24小时测量局部脑血流量（CBF）。在上矢状窦闭塞后约48小时，通过用4%多聚甲醛溶液灌注固定以及苏木精和伊红组织学检查来终止实验设置。
在测量时间段内，受闭塞影响平面内约45%的脑区CBF显著降低（p < 0.05）。组织学上，所有病例均显示亚急性静脉梗死的迹象。
基于新建立的猫上矢状窦显微结扎模型，我们首次提出了一种脑静脉梗死动物模型，该模型可导致经组织学证实的亚急性静脉梗死，且具有良好的可重复性。该模型的另一个优点是，其静脉梗死范围在个体间和个体内的差异以及CBF在24小时内缓慢降低的情况尽可能模拟了临床情况。连续PET成像为进一步深入了解实验性脑静脉梗死的病理生理特征提供了一种有利的非侵入性方法。因此，本研究中展示的新建立的猫模型对于脑静脉梗死的进一步详细研究以及治疗策略的实验评估具有重要价值。
