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Detection of hepatitis B virus DNA in human serum samples: use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction.

作者信息

Escarceller M, Rodriguez-Frias F, Jardi R, San Segundo B, Eritja R

机构信息

Department of Molecular Genetics, CID-CSIC, Barcelona, Spain.

出版信息

Anal Biochem. 1992 Oct;206(1):36-42. doi: 10.1016/s0003-2697(05)80007-7.

DOI:10.1016/s0003-2697(05)80007-7
PMID:1456439
Abstract

Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5'-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates.

摘要

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