DNA-肽核酸嵌合体的合成与DNA结合特性

Synthesis and DNA binding properties of DNA-PNA chimeras.

作者信息

Barone G, De Napoli L, Di Fabio G, Erra E, Giancola C, Messere A, Montesarchio D, Petraccone L, Piccialli G

机构信息

Dipartimento di Chimica, Università degli Studi di Napoli Federico II, Napoli, Italy.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1089-91. doi: 10.1081/NCN-120022743.

DOI:10.1081/NCN-120022743

PMID:14565351

Abstract

A systematic study to evaluate the ability of 5'-DNA-3'-p-(N)-PNA-(C) chimeras to form triple helix structures has been undertaken. Preliminary results carried out on a 16-mer chimera with three PNA monomers at the 3'-end showed the formation of a stable DNA-PNA/DNA/DNA triplex, having similar conformational behaviour to a canonical DNA/DNA/DNA triplex.

摘要

已开展一项系统性研究，以评估5'-DNA-3'-p-(N)-PNA-(C)嵌合体形成三链螺旋结构的能力。对在3'-末端带有三个PNA单体的16聚体嵌合体进行的初步结果表明，形成了一种稳定的DNA-PNA/DNA/DNA三链体，其构象行为与典型的DNA/DNA/DNA三链体相似。