RNase H activation by stereoregular boranophosphate oligonucleotide.
作者信息
Wang Xin, Dobrikov Mikhail, Sergueev Dmitri, Shaw Barbara Ramsay
机构信息
Department of Chemistry, Duke University, Durham, North Carolina 27708-0346, USA.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1151-3. doi: 10.1081/NCN-120022823.
PMID:14565367
Abstract
A stereoregular all-(Sp)-boranophosphate oligodeoxyribonucleotide (BH3(-)-ODN) 15-mer was synthesized using an enzymatic approach. The BH3(-)-ODN formed a hybrid with the complementary RNA 15-mer and induced RNase H hydrolysis of the RNA strand at ODN concentrations as low as 10 nM at 37 degrees C, but with a lower efficiency than that of its natural phosphodiester analogue.
摘要
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