通过将小沟结合剂与寡核苷酸偶联来稳定DNA双螺旋和三螺旋
Stabilization of DNA double and triple helices by conjugation of minor groove binders to oligonucleotides.
作者信息
Boutorine A S, Ryabinin V A, Novopashina D S, Venyaminova A G, Hélène C, Sinyakov A S
机构信息
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U 565, CNRS UMR 8646, Paris Cedex, France.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1267-72. doi: 10.1081/NCN-120022943.
New conjugates containing two parallel or antiparallel carboxamide minor groove binders (MGB) attached to the same terminal phosphate of one oligonucleotide strand were synthesized. The conjugates interact with their target DNA stronger than the individual components. Effect of conjugated MGB on DNA duplex and triplex stability and their sequence specificity was demonstrated on the short oligonucleotide duplexes and on the triplex formed by model 16-mer oligonucleotide with HIV polypurine tract.
合成了新的共轭物，其包含连接到一条寡核苷酸链的同一末端磷酸基团上的两个平行或反平行的羧酰胺小沟结合剂（MGB）。这些共轭物与其靶DNA的相互作用比单个组分更强。在短寡核苷酸双链体以及由具有HIV多聚嘌呤序列的16聚体模型寡核苷酸形成的三链体上，证明了共轭MGB对DNA双链体和三链体稳定性及其序列特异性的影响。
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