Taranenko M V, Sumbatyan N V, Mtchedlidze M T, Kuznetsova S A
Chemistry Department, Moscow State University, Moscow, Russia.
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1505-7. doi: 10.1081/NCN-120023021.
New reactive analogs of substrates for DNA repair enzyme E. coli Fpg protein containing the residues of 8-oxoguanine and photoactivatable phenyl(trifluoromethyl)diazirine groups were synthesized. Their substrate properties were investigated. Using photocross-linking technique, we established the presence of contacts of two nucleosides located near the oxoG with amino acids from the Fpg protein. The cross-linking efficiency achieved 10%.
合成了含有8-氧代鸟嘌呤残基和可光活化的苯基（三氟甲基）二氮烯基团的大肠杆菌Fpg蛋白DNA修复酶底物的新型反应类似物。研究了它们的底物性质。利用光交联技术，我们确定了位于氧代鸟嘌呤附近的两个核苷与Fpg蛋白的氨基酸之间存在接触。交联效率达到了10%。
