腺苷-2'（3'）-脱氧-3'（2'）-三磷酸及其相关类似物作为磷酸盐供体替代ATP用于所有人类和黑腹果蝇脱氧核糖核苷激酶的能力。

Ability of adenosine-2'(3')-deoxy-3'(2')-triphosphates and related analogues to replace ATP as phosphate donor for all human and Drosphila melanogaster deoxyribonucleoside kinases.

作者信息

Krawiec Krzysztof, Kierdaszuk Borys, Kalinichenko Elena N, Rubinova Elena B, Mikhailopulo Igor A, Eriksson Staffan, Munch-Petersen Birgitte, Shugar David

机构信息

Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Warsaw, Poland.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1525-9. doi: 10.1081/NCN-120023026.

DOI:10.1081/NCN-120023026

PMID:14565458

Abstract

Six non-conventional adenosine-2'- and 3'-triphosphate analogues of ATP were tested as potential phosphate donors for all four human, and D. melanogaster, deoxyribonucleoside kinases. With dCK (only dAdo as acceptor), TK1, TK2 and dNK only 3'-deoxyadenosine-2'-triphosphate was an effective donor (5-60% that for ATP). With dCK (dCyd as acceptor) and dGK (dGuo as acceptor), sharing 45% sequence identity, donor activities ranged from 13 to 119% that for ATP. Products were 5'-phosphates. In some instances, kinetics are dependent on the nature of the acceptor, and donor and acceptors properties are mutually interdependent. Results are highly relevant to studies on the modes of interaction with the enzymes, and to interpretations of reported crystal structures of dCK and dNK with bound ligands.

摘要

对六种非传统的ATP腺苷-2'-和3'-三磷酸类似物作为所有四种人类及黑腹果蝇脱氧核糖核苷激酶的潜在磷酸盐供体进行了测试。对于dCK（仅以dAdo作为受体）、TK1、TK2和dNK，只有3'-脱氧腺苷-2'-三磷酸是有效的供体（为ATP的5-60%）。对于dCK（以dCyd作为受体）和dGK（以dGuo作为受体），它们有45%的序列同一性，供体活性范围为ATP的13%至119%。产物为5'-磷酸盐。在某些情况下，动力学取决于受体的性质，并且供体和受体的性质相互依存。这些结果与关于与酶相互作用模式的研究以及对报道的结合配体的dCK和dNK晶体结构的解释高度相关。