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耐药性莫洛尼氏鼠白血病病毒逆转录酶和Taq DNA聚合酶将（α-P-硼烷）-2'，3'-二脱氧胞苷5'-三磷酸掺入DNA中。

Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase.

作者信息

Dobrikov Mikhail I, Sergueeva Zinaida A, Shaw Barbara Ramsay

机构信息

Department of Chemistry, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708-0346, USA.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1651-5. doi: 10.1081/NCN-120023091.

DOI:10.1081/NCN-120023091
PMID:14565487
Abstract

The Rp-stereoisomer of 5'-(alpha-P-borano)triphosphates of 2'-deoxycytidine (Rp-dCTPalphaB) and 2',3'-dideoxycytidine (Rp-ddCTPalphaB) were synthesized. Their steady-state kinetics of incorporation by ddNTP-resistant enzymes, e.g., MMLV reverse transcriptase (RT) and Taq DNA polymerase, were investigated and compared with incorporation of dCTP and ddCTP. The alpha-boranophosphate substitution in ddCTP results in a 28-fold increase in efficiency of incorporation of the Rp-ddCTPalphaB isomer by MMLV RT, yet has minimal effect on the efficiency of incorporation by Taq DNA polymerase.

摘要

合成了2'-脱氧胞苷（Rp-dCTPαB）和2'，3'-二脱氧胞苷（Rp-ddCTPαB）的5'-（α-P-硼烷）三磷酸酯的Rp-立体异构体。研究了它们被抗ddNTP酶（如莫洛尼鼠白血病病毒逆转录酶（RT）和Taq DNA聚合酶）掺入的稳态动力学，并与dCTP和ddCTP的掺入情况进行了比较。ddCTP中的α-硼代磷酸取代使MMLV RT掺入Rp-ddCTPαB异构体的效率提高了28倍，但对Taq DNA聚合酶的掺入效率影响最小。

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