mRNA 5' 帽类似物中磷酸链长度对其与真核起始因子4E相互作用的影响。

Influence of the length of the phosphate chain in mRNA 5' cap analogues on their interaction with eukaryotic initiation factor 4E.

作者信息

Zuberek Joanna, Jemielity Jacek, Niedzwiecka Anna, Stepinski Janusz, Wyslouch-Cieszynska Aleksandra, Stolarski Ryszard, Darzynkiewicz Edward

机构信息

Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1707-10. doi: 10.1081/NCN-120023119.

DOI:10.1081/NCN-120023119

PMID:14565501

Abstract

The recognition of the 5'mRNA cap structure m7G(5')ppp(5')N by one of the components of the initiation translation machinery, the eIF4E factor, plays a pivotal role in regulation of the protein synthesis. In the present study we have shown two opposing roles of the cap phosphate chain in the specific eIF4E-cap interaction. The extension of the phosphate chain enhances the binding of the cap to the unphosphorylated eIF4E but destabilises the eIF4E-cap complex in case of the phosphorylated protein.

摘要

起始翻译机制的组成部分之一eIF4E因子对5'mRNA帽结构m7G(5')ppp(5')N的识别在蛋白质合成调控中起关键作用。在本研究中，我们展示了帽磷酸链在特定的eIF4E-帽相互作用中的两种相反作用。磷酸链的延长增强了帽与未磷酸化的eIF4E的结合，但在蛋白质磷酸化的情况下会使eIF4E-帽复合物不稳定。