酵母真核生物起始因子eIF4E与mRNA 5'帽类似物之间的相互作用不同于小鼠eIF4E的相互作用。

Interaction between yeast eukaryotic initiation factor eIF4E and mRNA 5' cap analogues differs from that for murine eIF4E.

作者信息

Kiraga-Motoszko Katarzyna, Stepinski Janusz, Niedzwiecka Anna, Jemielity Jacek, Wszelaka-Rylik Malgorzata, Stolarski Ryszard, Zielenkiewicz Wojciech, Darzynkiewicz Edward

机构信息

Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1711-4. doi: 10.1081/NCN-120023120.

DOI:10.1081/NCN-120023120

PMID:14565502

Abstract

Measurements of interaction of 7-methyl-GTP eIF4E from S. cerevisiae were performed by means of two methods: Isothermal Titration Calorimetry (ITC) and fluorescence titration. The equilibrium association constants (Kas) derived from the two methods show significantly different affinity of yeast eIF4E for the mRNA 5' cap than those of the murine and human proteins. The observed differences in the Kas values and the enthalpy changes of the association (deltaH(o)) suggest some dissimilarity in the mode of binding and stabilization of cap in the complexes with eIF4E from various sources.

摘要

通过两种方法对来自酿酒酵母的7-甲基-GTP eIF4E的相互作用进行了测量：等温滴定量热法（ITC）和荧光滴定法。从这两种方法得出的平衡缔合常数（Kas）表明，酵母eIF4E对mRNA 5'帽的亲和力与小鼠和人类蛋白质的亲和力有显著差异。观察到的Kas值差异以及缔合焓变（deltaH(o)）表明，在与来自不同来源的eIF4E形成的复合物中，帽的结合和稳定模式存在一些差异。