通过染料介导的光氧化稳定的牛颈静脉补片的体外内皮化。
In-vitro endothelialization of patches of bovine jugular vein stabilized by dye-mediated photooxidation.
作者信息
Li Shengli, Hu Shengshou, Zhou Jianye, Liao Zhongkai
机构信息
Division of Cardiac Valvular Prosthesis and Circulation Assistance, Department of Surgery, Fuwai Heart Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, Peoples' Republic of China.
出版信息
J Heart Valve Dis. 2003 Sep;12(5):625-7.
BACKGROUND AND AIM OF THE STUDY
The study aim was to evaluate the cytotoxicity of photooxidatively stabilized bovine jugular vein and the in-vitro endothelialization properties of such material.
METHODS
Bovine jugular veins were cross-linked by dye-mediated photooxidation and cut into 4 cm2 pieces. Ovine jugular vein endothelial cells were isolated, cultured, and then seeded onto the inner surface of bovine jugular vein patches at a density of 1.5 x 10(5) per cm2. Specimens were cultured for five days and then examined using scanning electron microscopy (SEM) and immunohistochemical staining for von Willebrand factor.
RESULTS
SEM showed confluent lining of cultured ovine jugular vein endothelial cells similar to native endothelium. Von Willebrand factor staining for cells on the surface of patches was positive.
CONCLUSION
The photooxidatively stabilized bovine jugular vein patches were not cytotoxic, and their in-vitro endothelialization was possible.
研究背景与目的
本研究旨在评估光氧化稳定化牛颈静脉的细胞毒性以及该材料的体外内皮化特性。
方法
牛颈静脉通过染料介导的光氧化进行交联，然后切成4平方厘米的片。分离、培养绵羊颈静脉内皮细胞，接着以每平方厘米1.5×10⁵个细胞的密度接种到牛颈静脉补片的内表面。标本培养五天，然后使用扫描电子显微镜（SEM）和针对血管性血友病因子的免疫组织化学染色进行检查。
结果
SEM显示培养的绵羊颈静脉内皮细胞形成融合衬里，类似于天然内皮。补片表面细胞的血管性血友病因子染色呈阳性。
结论
光氧化稳定化的牛颈静脉补片无细胞毒性，且其体外内皮化是可行的。
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