Li Zi-Qiang, Wu Zhong-Hai, Shi Yue, Wang Ning-Qian
Department of Physiology, The First Military Medical University, Guangzhou 510515.
Sheng Li Xue Bao. 2003 Oct 25;55(5):560-4.
This experiment was expected to test whether nitric oxide (NO) exerted significant effect on the central respiratory rhythm. Experiments were performed on in vitro brainstem slice preparations from neonatal rats. These preparations include the medial region of the nucleus retrofacialis (mNRF); a part of pre-Bötinger complex, ventral respiratory group (VRG) and dorsal respiratory group (DRG). Respiratory-related burst activities were recorded from hypoglossal nerve rootlets before and during superfusion of the slice preparation with L-Arginine (L-Arg), sodium nitroprusside (SNP) or 7-nitro indazole (7-NI, an inhibitor of NO synthase). After perfusion with L-Arg and SNP, there was no significant change in respiratory rhythmical discharge activity (RRDA), but 7-NI decreased the integral amplitude of burst and inspiratory time. These results indicate that NO may take part in the inspiratory off-switching mechanism and that it also modulates the amplitude of respiratory-related bursts.
本实验旨在测试一氧化氮（NO）是否对中枢呼吸节律有显著影响。实验采用新生大鼠的离体脑干切片标本进行。这些标本包括面神经后核内侧区域（mNRF）；前包钦格复合体的一部分、腹侧呼吸组（VRG）和背侧呼吸组（DRG）。在向切片标本灌注L-精氨酸（L-Arg）、硝普钠（SNP）或7-硝基吲唑（7-NI，一种一氧化氮合酶抑制剂）之前和期间，记录舌下神经根的呼吸相关爆发活动。灌注L-Arg和SNP后，呼吸节律性放电活动（RRDA）无显著变化，但7-NI降低了爆发的积分幅度和吸气时间。这些结果表明，NO可能参与吸气切断机制，并且它还调节呼吸相关爆发的幅度。
