手术期间循环MN/CA9阳性肾癌细胞的检测

Detection of circulating MN/CA9 positive renal cell carcinoma cells during operation.

作者信息

Uemura H, Nakagawa Y, Iwai A, Okajima E, Okajima E, Yoshikawa K, Hirao Y

机构信息

Department of Urology, Nara Medical University, Kashihara, Japan.

出版信息

Aktuelle Urol. 2003 Jul;34(4):270-2. doi: 10.1055/s-2003-41615.

DOI:10.1055/s-2003-41615

PMID:14566683

Abstract

Our previous study demonstrated the clear detection of MN/CA9 mRNA in peripheral blood samples from RCC patients. However, approximately 30 % of control blood samples from healthy volunteers showed MN/CA9 expression. We have developed a new primer set and optimized the PCR conditions, now resulting in a specificity of 100 %. In tissue samples, all clear cell type carcinomas but none of the spindle cell type and pleomorphic cell type tumors expressed the MN/CA9 message. Analysis of MN/CA9 messages in peripheral blood samples from RCC patients gave positive results for 0/2, 1/9, 0/4 and 4/12 of stage I, II, III and IV cases, respectively. RT-PCR analysis using preoperative renal venous blood samples resulted in clear detection of MN/CA9 positive cells in 2/4, 3/13, 2/6 and 1/1 of stage I, II, III and IV cases, respectively. Our results suggest that assessment of MN/CA9 expression by RT-PCR is a promising method for detecting cancer cells in the circulation of patients with RCC.

摘要

我们之前的研究表明，在肾细胞癌（RCC）患者的外周血样本中可清晰检测到MN/CA9 mRNA。然而，约30%来自健康志愿者的对照血样显示有MN/CA9表达。我们开发了一种新的引物组并优化了PCR条件，现在特异性达到了100%。在组织样本中，所有透明细胞型癌均表达MN/CA9信息，但梭形细胞型和多形细胞型肿瘤均未表达。对RCC患者外周血样本中的MN/CA9信息进行分析，I期、II期、III期和IV期病例的阳性结果分别为0/2、1/9、0/4和4/12。使用术前肾静脉血样本进行RT-PCR分析，I期、II期、III期和IV期病例中MN/CA9阳性细胞的清晰检测结果分别为2/4、3/13、2/6和1/1。我们的结果表明，通过RT-PCR评估MN/CA9表达是检测RCC患者循环中癌细胞的一种有前景的方法。