Fluorescent complex of pyoverdin with aluminum.
作者信息
del Olmo A, Caramelo C, SanJose C
机构信息
Departamento de Nutrición y Bromatologi;a III, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040, Madrid, Spain.
出版信息
J Inorg Biochem. 2003 Dec 1;97(4):384-7. doi: 10.1016/s0162-0134(03)00316-7.
Abstract
When a pyoverdin (PV), (a siderophore) from Pseudomonas fluorescens, binds aluminum 1:1, its natural fluorescence almost doubles, whereas PV-Fe is non-fluorescent. Complex formation allows [Al] determination down to 1 mug/l. Fe(III) in the sample interferes with [Al] determination, but added after PV, improves the assay's performance. Ascorbic acid does not eliminate Fe(III) interference. PV-Al fluorescence could have analytical and toxicological applications.
摘要
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