Turgut Günfer, Demir Süleyman, Genç Osman, Karabulut Ismail, Akalin Nalan
Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Acta Physiol Pharmacol Bulg. 2003;27(2-3):43-5.
We intended to study the effect of swimming exercise on the brain, liver and heart malondialdehyde (MDA) levels which are the last product of oxidation, and to compare them with the brain, liver and heart MDA levels of controls. The experiments were carried out on 20 Wistar rats which were fed with a standard laboratory chow diet ad libitum. Rats were distributed in two groups, control group (n = 10) and exercise group (n = 10). The exercise group rats were exposed to swimming exercise for 30 minutes. After this animals in each group were sacrificed by decapitation, their brain, liver and heart tissues were quickly removed. MDA levels of the brain, liver and heart were determined according to the method in which MDA reacts with thiobarbituric acid. Results were evaluated by the Mann-Whitney U test. The liver and heart MDA levels in the exercise group were (29.59+/-6.73 and 10.49+/-1.90 nmol/g tissue, respectively) significantly higher than in the control group (21.78+/-3.46 and 8.86+/-1.25 nmol/g tissue, p<0.01 and p<0.05, respectively). However, the brain MDA levels were similar in both groups (exercise group 19.37+/-5.50 nmol/g tissue and control group 16.58+/-2.44 nmol/g tissue; p=0.325). It is concluded that swimming exercise might cause oxidative stress.
我们旨在研究游泳锻炼对大脑、肝脏和心脏中丙二醛（MDA）水平的影响，丙二醛是氧化的终产物，并将其与对照组的大脑、肝脏和心脏MDA水平进行比较。实验在20只Wistar大鼠上进行，这些大鼠随意喂食标准实验室饲料。大鼠被分为两组，对照组（n = 10）和运动组（n = 10）。运动组大鼠进行30分钟的游泳锻炼。之后，每组动物通过断头处死，迅速取出它们的大脑、肝脏和心脏组织。根据丙二醛与硫代巴比妥酸反应的方法测定大脑、肝脏和心脏的MDA水平。结果通过Mann-Whitney U检验进行评估。运动组的肝脏和心脏MDA水平（分别为29.59±6.73和10.49±1.90 nmol/g组织）显著高于对照组（21.78±3.46和8.86±1.25 nmol/g组织；分别为p<0.01和p<0.05）。然而，两组的大脑MDA水平相似（运动组为19.37±5.50 nmol/g组织，对照组为16.58±2.44 nmol/g组织；p = 0.325）。结论是游泳锻炼可能会导致氧化应激。
