Mjøs Svein A
Norwegian Institute of Fisheries and Aquaculture Research, Kjerreidviken 16, N-5141 Fyllingsdalen, Norway.
J Chromatogr A. 2003 Oct 10;1015(1-2):151-61. doi: 10.1016/s0021-9673(03)01240-8.
A method for the identification of fatty acid methyl esters (FAME) based on the analysis of shifts in equivalent chain lengths (ECL) is described. The method is based on two-dimensional retention data achieved on one capillary column. Various temperature and pressure programs are applied on the same cyanopropyl column and the shifts in the ECL values are analysed by multivariate methods. The chain length, number of double bonds, and the double bond positions can be determined with high accuracy. The same procedure is suitable for determination of the number of trans and cis double bonds in trans fatty acids, and for detection of artefacts in fatty acid methyl ester chromatograms.
描述了一种基于等效链长（ECL）变化分析来鉴定脂肪酸甲酯（FAME）的方法。该方法基于在一根毛细管柱上获得的二维保留数据。在同一氰丙基柱上应用各种温度和压力程序，并通过多变量方法分析ECL值的变化。可以高精度地确定链长、双键数量和双键位置。相同的程序适用于测定反式脂肪酸中反式和顺式双键的数量，以及检测脂肪酸甲酯色谱图中的假象。
