在感染小肠结肠炎耶尔森氏菌O8血清型的小鼠中检测N-(3-氧代己酰基)-L-高丝氨酸内酯。
Detection of N-(3-oxohexanoyl)-L-homoserine lactone in mice infected with Yersinia enterocolitica serotype O8.
作者信息
Jacobi Christoph A, Bach Alexandra, Eberl Leo, Steidle Anette, Heesemann Jürgen
机构信息
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximillian University Munich, D-80336 Munich, Germany.
出版信息
Infect Immun. 2003 Nov;71(11):6624-6. doi: 10.1128/IAI.71.11.6624-6626.2003.
Abstract
Yersinia enterocolitica synthesizes N-acyl-L-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-L-homoserine lactone] and not HHL (N-hexanoyl-L-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.
摘要
小肠结肠炎耶尔森菌通过LuxR-LuxI同源物YenR-YenI合成N-酰基-L-高丝氨酸内酯（AHL）信号分子。在本研究中，我们检测了两株对小鼠具有毒力的小肠结肠炎耶尔森菌O8血清型菌株WA-314和8081在体外和体内（小鼠感染模型）产生AHL的情况。我们使用薄层色谱法结合大肠杆菌AHL生物传感器来鉴定所产生的AHL种类。我们在培养上清液或感染小鼠组织中仅检测到小肠结肠炎耶尔森菌O8产生的OHHL [N-（3-氧代己酰基）-L-高丝氨酸内酯]，而未检测到HHL（N-己酰基-L-高丝氨酸内酯）。这是第一份证明耶尔森菌在感染过程中产生AHL的报告。
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