用于研究N-酰基高丝氨酸内酯介导的群体感应的基于luxCDABE的质粒传感器的构建与分析。
Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing.
作者信息
Winson M K, Swift S, Fish L, Throup J P, Jørgensen F, Chhabra S R, Bycroft B W, Williams P, Stewart G S
机构信息
Food Microbiology Section, School of Biological Sciences, University of Nottingham, Leics, UK.
出版信息
FEMS Microbiol Lett. 1998 Jun 15;163(2):185-92. doi: 10.1111/j.1574-6968.1998.tb13044.x.
Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.
已经构建了质粒报告载体，这些载体可响应N-酰基高丝氨酸内酯（AHLs）对LuxR及其同源物LasR和RhlR（VsmR）的激活。luxCDABE分别与PluxI、PlasI和PrhlI转录融合，在存在激活AHLs的情况下表达。可以看到一种结构/活性关系概况，其中天然配体最有效。对单个LuxR同源物/AHL组合的表征允许对来自测试生物体的群体感应信号进行全面评估。
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