Sim Seobo, Park Gab-Man, Yong Tai-Soon
Department of Parasitology and Institute of Tropical Medicine, BK21 Project for Medical Science, Yonsei University College of Medicine, 120-752, Seoul, Korea.
Parasitol Res. 2003 Oct;91(4):338-43. doi: 10.1007/s00436-003-0869-7. Epub 2003 Sep 16.
Clonorchis sinensis excretory-secretory (ES) antigens were separated by gradient SDS-PAGE and the antigenic protein isolated at 21 kDa was injected into rats. A C. sinensis cDNA library was then immunoscreened with sera from the 21-kDa antigenic protein-immunized rats. The selected genes, which were named C. sinensis myoglobin, contained a single open reading frame of 450 base pairs encoding 150 amino acids. A single hybridized band of 0.57 kb and two to four hybridized bands were detected by Northern and Southern blotting. Purified recombinant C. sinensis myoglobin was recognized by clonorchiasic rabbit sera (50%) and clonorchiasic human sera (25%). An indirect immunofluorescence assay showed that C. sinensis myoglobin is distributed over the whole body of the adult worm. It is believed that the abundance of C. sinensis myoglobin plays an important role as an oxygen reservoir under anaerobic conditions.
华支睾吸虫排泄分泌（ES）抗原经梯度SDS-PAGE分离，将分离出的21 kDa抗原性蛋白注射到大鼠体内。随后用来自21 kDa抗原性蛋白免疫大鼠的血清对华支睾吸虫cDNA文库进行免疫筛选。所选基因命名为华支睾吸虫肌红蛋白，包含一个450个碱基对的单一开放阅读框，编码150个氨基酸。通过Northern和Southern印迹检测到一条0.57 kb的单一杂交带以及两到四条杂交带。纯化的重组华支睾吸虫肌红蛋白可被华支睾吸虫病兔血清（50%）和华支睾吸虫病人血清（25%）识别。间接免疫荧光试验表明，华支睾吸虫肌红蛋白分布于成虫全身。据信，华支睾吸虫肌红蛋白的丰富含量在厌氧条件下作为氧储备发挥重要作用。
