Wang Seu-Mei, Hwang Ran-Der, Greenberg Andrew S, Yeo Hui-Ling
Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, 1-1 Jen-Ai Road, 100 Taipei, Taiwan.
Histochem Cell Biol. 2003 Oct;120(4):285-92. doi: 10.1007/s00418-003-0575-7. Epub 2003 Sep 20.
This study aimed to investigate the relationship between newly formed lipid droplets and lipid droplet surface proteins, including perilipin, adipose differentiation related protein (ADRP), and p200 kDa protein (p200) in 3T3-L1 preadipocytes, during lipogenesis. Sterol ester was used to induce nascent lipid droplets in 3T3-L1 preadipocytes and the sequence of lipids and lipid droplet surface proteins was studied using a combination of immunohistochemistry and Nile red staining/Oil red O. We demonstrated that, although most growing lipid droplets appeared to have a lipid core surrounded by a fluorescent rim of ADRP, perilipin, and p200, tiny protein aggregates of ADRP, perilipin, or p200 could also be found to occur in the absence of lipid accumulation. In addition, ADRP associated with nascent lipid droplets prior to that of perilipin or p200. We provide evidence that lipid droplet surface proteins, especially ADRP and perilipin, are important in serving as a nucleation center for the assembly of lipid to form nascent lipid droplets.
本研究旨在探讨在脂肪生成过程中，3T3-L1前脂肪细胞中新形成的脂滴与脂滴表面蛋白（包括围脂滴蛋白、脂肪分化相关蛋白（ADRP）和200 kDa蛋白（p200））之间的关系。使用甾醇酯诱导3T3-L1前脂肪细胞中新生脂滴的形成，并结合免疫组织化学和尼罗红染色/油红O研究脂质和脂滴表面蛋白的序列。我们证明，尽管大多数生长中的脂滴似乎有一个由ADRP、围脂滴蛋白和p200的荧光边缘包围的脂质核心，但在没有脂质积累的情况下也能发现ADRP、围脂滴蛋白或p200的微小蛋白聚集体。此外，ADRP在围脂滴蛋白或p200之前与新生脂滴相关联。我们提供的证据表明，脂滴表面蛋白，尤其是ADRP和围脂滴蛋白，在作为脂质组装形成新生脂滴的成核中心方面很重要。
