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用于检测禽类血清中H7流感抗体的酶联免疫吸附测定(ELISA)试验。

ELISA test for the detection of influenza H7 antibodies in avian sera.

作者信息

Sala G, Cordioli P, Moreno-Martin A, Tollis M, Brocchi E, Piccirillo A, Lavazza A

机构信息

Istituto Zooprofilattico della Lombardia ed Emilia Romagna B.Ubertini Via A. Bianchi, 9-25124 Brescia, Italy.

出版信息

Avian Dis. 2003;47(3 Suppl):1057-9. doi: 10.1637/0005-2086-47.s3.1057.

DOI:10.1637/0005-2086-47.s3.1057
PMID:14575110
Abstract

Using a monoclonal antibody (MAb) specific for the H7 influenza surface glycoproteins, a serological enzyme-linked immunosorbent assay (ELISA) test has been developed. This MAb was made using the low-pathogenicity (LP) avian influenza (AI) strain (BS2676/99) isolated in Italy during a recent outbreak. The test is able to detect H7 antibodies in avian sera. The H7 ELISA has a 99% concordance of results with the classical hemagglutination inhibition (HI) test.

摘要

利用一种针对H7流感表面糖蛋白的单克隆抗体(MAb),开发了一种血清学酶联免疫吸附测定(ELISA)试验。这种单克隆抗体是使用在最近一次疫情期间于意大利分离出的低致病性(LP)禽流感(AI)毒株(BS2676/99)制备的。该试验能够检测禽类血清中的H7抗体。H7 ELISA试验结果与经典血凝抑制(HI)试验的结果一致性达99%。

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