Plasticity of synaptic ribbons of the rat pineal gland in vitro--minor effects of electrical stimulation.

Synaptic ribbons (SRs) of mammalian pinealocytes exhibit day/night changes in number and size, changes that are apparently regulated by the suprachiasmatic nucleus via postganglionic sympathetic nerve fibres. Since the neural control of SR changes is far from clear and as pinealocytes produce action potentials, we undertook to investigate whether electrical stimulation affects SR changes. Isolated rat pineal glands removed during the daytime were kept in vitro for 0, 30, 60, 90 or 120 min, with or without continuous electrical stimulation (1 mA, 1 Hz), followed by the quantification of SR profiles (SRPs) by transmission electron microscopy. SRs were categorised as to whether they lay less than 100 nm away from the pinealocyte plasmalemma (SRPs(near)) or more distant from it (SRPs(dist)) and the lengths of the profiles were measured. Cultured pineal organs showed a significant numerical depression of SRPs(near), irrespective of whether the organs had been electrically stimulated or not. SRPs(near) length revealed a significant increase at 60 min in unstimulated control tissue and at 30 min in electrically stimulated glands. SRPs(dist) length decreased significantly at 30 min in control glands and after 60 min in electrically stimulated glands. Thus, action potentials inside the pineal gland appear to be minor factors regulating SR numbers. In future pineal studies, SRPs(near) and SRPs(dist) should be considered separately as they differ in plasticity.