Pan Yang-lin, Bi Feng, Liu Na, Du Jing-ping, Jiang Hai-ping, Xue Yan, Fan Dai-ming
Institute of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China.
Zhonghua Zhong Liu Za Zhi. 2003 Sep;25(5):441-4.
To investigate the significance of Rac subfamily members in the gastrointestinal carcinogenesis and progression.
The mRNA expression of Rac1, Rac2 and Rac3 in 12 kinds of gastrointestinal cancer cell lines was examined by semi-quantitative RT-PCR. The activities of Rac1 protein in 5 kinds of gastric cancer cell lines were tested by pull-down assay.
Compared with the normal gastric mucosa and intestinal epithelial cell line, the mRNA expression of Rac1 and Rac3 was up-regulated in most of gastrointestinal cancer cell lines. The activities of Rac1 protein increased markedly in gastric cancer cell lines.
The increased mRNA expression of Rac1 and Rac3 in gastrointestinal cancer cell lines and the abnormal activation of Rac1 protein in gastric cancer cell lines might be correlated with the carcinogenesis of gastrointestinal cancer.
探讨Rac亚家族成员在胃肠道癌发生及进展中的意义。
采用半定量逆转录聚合酶链反应（RT-PCR）检测12种胃肠道癌细胞系中Rac1、Rac2和Rac3的mRNA表达。采用下拉分析法检测5种胃癌细胞系中Rac1蛋白的活性。
与正常胃黏膜和肠上皮细胞系相比，大多数胃肠道癌细胞系中Rac1和Rac3的mRNA表达上调。胃癌细胞系中Rac1蛋白的活性显著增加。
胃肠道癌细胞系中Rac1和Rac3的mRNA表达增加以及胃癌细胞系中Rac1蛋白的异常激活可能与胃肠道癌的发生有关。
