Lu Shi-hong, Feng Yi, Yang Ren-chi, Liu Yong-jun, Zhai Qiong-li, Zhang Zhi-hua, Han Zhong-chao
State key Laboratory of Experimental Hematology, Institute of Hematology, CAMS & PUMC, Tianjin 300020, China.
Zhonghua Xue Ye Xue Za Zhi. 2003 Sep;24(9):467-9.
To investigate the effects of platelet factor 4 (PF4) on the adherence, and the expressions of adherent molecules CD(49d) and CXCR4 and the receptor of SDF-1 of fresh and expanded cord blood CD(34)(+) cells.
CD(34)(+) cells were isolated from cord blood using MACS immune magnetic beads. The adherent ability was assayed by using crystal violet staining and the expression of adherent molecule CD(49d) and CXCR4 by FACS.
(1) PF4 could increase the adherent ability of the fresh cord blood CD(34)(+) cells, the effect being positively correlated with the dose of PF4. (2) SDF-1 at concentration of 100 ng/ml increased the adherent ability of the fresh cord blood CD(34)(+) cells. (3) The spontaneous and the SDF-1 induced adherent ability of the cord blood CD(34)(+) cells began to decrease after being cultured for 10 days without PF4, while in the presence of PF4 at 100 ng/ml, the ability of the cord blood CD(34)(+) cell adhering to the stroma layer still remained at higher level. At day 14, the adherent ability was (262.04 +/- 64.81)% and (64.35 +/- 8.29)% in PF4 group and control group, respectively, if it was defined as 100% at day 0. SDF-1 at concentration of 100 ng/ml induced adherent ability was (138.31 +/- 32.39)% and (67.66 +/- 12.44)% in PF4 group and control group, respectively. (4) The expression of CD(49d) and CXCR4 increased 13.02% and 17.33%, respectively, when incubated with PF4.
PF4 could increase the adherent ability and promote the expression of CD(49d) and CXCR4 of the cord blood CD(34)(+) cells, suggesting that PF4 promote the circulating stem cells homing to the marrow in the process of stem cells transplantation.
探讨血小板因子4（PF4）对新鲜和扩增脐血CD(34)(+)细胞黏附能力、黏附分子CD(49d)和CXCR4以及基质细胞衍生因子-1（SDF-1）受体表达的影响。
采用MACS免疫磁珠法从脐血中分离CD(34)(+)细胞。通过结晶紫染色检测黏附能力，采用流式细胞术检测黏附分子CD(49d)和CXCR4的表达。
（1）PF4可增强新鲜脐血CD(34)(+)细胞的黏附能力，且该作用与PF4剂量呈正相关。（2）浓度为100 ng/ml的SDF-1可增强新鲜脐血CD(34)(+)细胞的黏附能力。（3）在无PF4培养10天后，脐血CD(34)(+)细胞的自发黏附能力及SDF-1诱导的黏附能力开始下降，而在100 ng/ml PF4存在的情况下，脐血CD(34)(+)细胞与基质层的黏附能力仍维持在较高水平。第14天时，若将第0天的黏附能力定义为100%，PF4组和对照组的黏附能力分别为（262.04±64.81）%和（64.35±8.29）%。浓度为100 ng/ml的SDF-1诱导的黏附能力在PF4组和对照组分别为（138.31±32.39）%和（67.66±12.44）%。（4）与PF4孵育后，CD(49d)和CXCR4的表达分别增加了13.02%和17.33%。
PF4可增强脐血CD(34)(+)细胞的黏附能力，促进CD(49d)和CXCR4的表达，提示PF4在干细胞移植过程中促进循环干细胞归巢至骨髓。
