Feng Yi, Lu Shi-hong, Song Guo-li, Zhang Zhi-hua, Han Zhong-chao
State key Laboratory of Experimental Hematology, Institute of Hematology, National Research Center of Stem Cell Engineering & Technology, CAMS & PUMC, Tianjin 300020, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 May;19(2):141-4.
To study the effect of PF4 and relative peptide PF4 17-70 on the chemoattract ability, the expression of adhesion molecules and CXCR4 on the flesh cord blood CD34+ cells.
CD34+ cells were separated from the cord blood using MACS immune magnetic beads, the chemoattract ability was assayed using the Transwell board, the expression of adhesion molecules and CXCR4 was measured by FACS.
(1) PF4 and PF4 17-70 increased the migration of the CD34+ cells, the chemoattract percentage of PF4 was 157.43% +/- 50.06% (P < 0.05) and PF4 17-70 was 187.02% +/- 10.69% (P < 0.05). (2) The expression of CD49d and CXCR4 on the CD34+ cells increased after PF4 incubated, but the expressions of other adherent molecules including CD31, CD44, CD11a, CD62p, CD62E did not change.
PF4 has the chemoattract ability on the umbilical blood CD34+ cells by promoting the expression of integrin CD49d and CXCR4, PF4 may help the cord stem cells homing.
研究血小板第4因子（PF4）及其相关肽PF4 17 - 70对脐血CD34+细胞趋化能力、黏附分子及趋化因子受体4（CXCR4）表达的影响。
采用磁珠分选法从脐血中分离CD34+细胞，用Transwell小室检测其趋化能力，用流式细胞术检测黏附分子及CXCR4的表达。
（1）PF4和PF4 17 - 70可增加CD34+细胞的迁移，PF4的趋化百分率为157.43%±50.06%（P<0.05），PF4 17 - 70为187.02%±10.69%（P<0.05）。（2）PF4作用后，CD34+细胞上CD49d和CXCR4的表达增加，而CD31、CD44、CD11a、CD62p、CD62E等其他黏附分子的表达无变化。
PF4可通过促进整合素CD49d和CXCR4的表达，对脐血CD34+细胞产生趋化作用，可能有助于脐血干细胞归巢。
