Lu Lu, Hou Ming, Shi Yan, Qin Ping, Peng Jun, Zhu Yuan-yuan, Zhang Mao-hong
Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Zhonghua Xue Ye Xue Za Zhi. 2003 Sep;24(9):477-9.
To investigate a specific and sensitive assay for the diagnosis of autoimmune thrombocytopenic purpura (AITP).
Glycoprotein specific autoantibodies in platelet eluate and plasma were detected by a modified monoclonal antibody immobilization of platelet antigens assay (MAIPA).
The overall positive rate of specific autoantibodies against platelet GPIIb/IIIa and GPIb/IX in plasma was 38.89% and in eluated platelet membrane was 68.52%. The difference between them was significant (corrected chi(2) = 19.39, P < 0.005). The proportion of positive MAIPA results between primary AITP and secondary AITP was not significantly different. There was a significant inverse correlation between antibody level and platelet count.
Detection of eluated GP-specific autoantibodies by MAIPA is highly specific and much more sensitive as compared with the measurement of their plasma counterparts in the diagnosing and therapeutic monitoring of AITP.
研究一种用于诊断自身免疫性血小板减少性紫癜（AITP）的特异且灵敏的检测方法。
采用改良的血小板抗原单克隆抗体固定试验（MAIPA）检测血小板洗脱液和血浆中的糖蛋白特异性自身抗体。
血浆中针对血小板糖蛋白IIb/IIIa和GPIb/IX的特异性自身抗体总体阳性率分别为38.89%和68.52%，两者差异有统计学意义（校正卡方值=19.39，P<0.005）。原发性AITP和继发性AITP之间MAIPA阳性结果比例差异无统计学意义。抗体水平与血小板计数之间存在显著负相关。
在AITP的诊断和治疗监测中，与检测血浆中的糖蛋白特异性自身抗体相比，采用MAIPA检测洗脱的糖蛋白特异性自身抗体具有更高的特异性和敏感性。
