[端粒酶逆转录酶在癌前食管鳞状上皮发育异常中的表达]

[Expression of telomerase reverse transcriptase in premalignant esophageal squamous dysplasia].

作者信息

Yu Hong-Ping, Xu Shun-Qing, Liu Li, Shi Lu-Yuan, Lu Wen-Hong

机构信息

Institute of Environmentanl Medicine, TongJi Medical College, Huazhong Science and Technology University, Wuhan 430030, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Oct;24(10):920-3.

PMID:14575609

Abstract

OBJECTIVE

To study the relationship of human telomerase reverse transcriptase (hTRT) and malignant transformation of esophageal dysplasia.

METHODS

Telomerase activity and hTRT expression in esophageal dysplasia (n = 47), squamous cell carcinoma (n = 29) and normal esophagus (n = 11) were detected by telomeric repeat amplification protocol (TRAP) and in situ hybridization, respectively.

RESULTS

Telomerase activity was detected in none of the 11 cases of normal esophageal tissues (0%) but in 21 of 47 cases (44.7%) of dysplasia, and in 25 of 29 cases (86.2%) of esophageal squamous cell carcinoma. There were statistically significant differences among the telomerase activity in normal esophagus, esophageal dysplasia, and in squamous cell carcinoma (chi(2) = 5.89, P < 0.05; chi(2) = 11.35, P < 0.01). hTRT mRNA was expressed in none of the 11 cases of normal esophageal tissues (0%) but in 23 of 47 cases (48.9%) of dysplasia, and in 24 of 29 cases (82.8%) of esophageal squamous cell carcinoma. There were statistically significant differences among the expression of hTRT mRNA in normal esophagus, esophageal dysplasia, and in squamous cell carcinoma (chi(2) = 6.99, P < 0.01; chi(2) = 7.32, P < 0.01). Significant correlation was found between the telomerase activity and the expression of hTRT mRNA (chi(2) = 57.91, P < 0.001).

CONCLUSION

The mRNA expression of hTRT which paralleled to telomerase activity implied that there was a crucial role to play in regulating the activation of telomerase, and was closely related to the malignant transformation of esophageal dysplasia. hTRT might serve as a new, valuable biomarker to detect esophageal squamous cell carcinoma.

摘要

目的

研究人端粒酶逆转录酶（hTRT）与食管发育异常恶变的关系。

方法

分别采用端粒重复序列扩增法（TRAP）和原位杂交技术检测食管发育异常（n = 47）、鳞状细胞癌（n = 29）及正常食管组织（n = 11）中的端粒酶活性和hTRT表达。

结果

11例正常食管组织中均未检测到端粒酶活性（0%），但47例发育异常组织中有21例（44.7%）检测到，29例食管鳞状细胞癌中有25例（86.2%）检测到。正常食管、食管发育异常及鳞状细胞癌中端粒酶活性存在统计学差异（χ² = 5.89，P < 0.05；χ² = 11.35，P < 0.01）。11例正常食管组织中均未检测到hTRT mRNA表达（0%），但47例发育异常组织中有23例（48.9%）检测到，29例食管鳞状细胞癌中有24例（82.8%）检测到。正常食管、食管发育异常及鳞状细胞癌中hTRT mRNA表达存在统计学差异（χ² = 6.99，P < 0.01；χ² = 7.32，P < 0.01）。端粒酶活性与hTRT mRNA表达之间存在显著相关性（χ² = 57.91，P < 0.001）。