Rodriguez-Cea A, Ayllon F, Garcia-Vazquez E
Departamento de Biologia Funcional, Universidad de Oviedo, C/Julián Claveri;a s/n, 33006, Oviedo, Spain.
Ecotoxicol Environ Saf. 2003 Nov;56(3):442-8. doi: 10.1016/s0147-6513(03)00073-3.
Brown trout, Salmo trutta, European eel, Anguilla anguilla, and European minnow, Phoxinus phoxinus, three fish species inhabiting European freshwater ecosystems, were evaluated for their use as in situ pollution biomarkers using the micronucleus test in renal erythrocytes. Experimental exposure (by immersion) to different concentrations of cyclophosphamide, colchicine, and cadmium showed that brown trout are more sensitive to the three compounds than minnows and eels. In situ surveys of wild freshwater ecosystems with different levels of pollution showed that minnows and eels living in polluted sites do not present higher micronuclei averages than those caught in clean rivers systems, whereas micronuclei are induced in brown trout inhabiting polluted sites. Our results demonstrated the suitability of brown trout for in situ biomonitoring of freshwater ecosystems as well as for laboratory tests using the micronucleus test.
褐鳟（Salmo trutta）、欧洲鳗鲡（Anguilla anguilla）和欧洲 minnows（Phoxinus phoxinus）是栖息于欧洲淡水生态系统的三种鱼类，通过对其肾红细胞进行微核试验，评估它们作为原位污染生物标志物的适用性。通过浸泡对不同浓度的环磷酰胺、秋水仙碱和镉进行实验暴露，结果表明，褐鳟对这三种化合物的敏感性高于 minnows 和鳗鱼。对不同污染水平的野生淡水生态系统进行的原位调查表明，生活在污染场地的 minnows 和鳗鱼的微核平均水平并不高于在清洁河流系统中捕获的同类，而栖息在污染场地的褐鳟会诱发微核。我们的结果证明了褐鳟适用于淡水生态系统的原位生物监测以及使用微核试验的实验室测试。
