Distasi Carla, Gilardino Alessandra, Erriquez Jessica, Zamburlin Pollyanna, Lovisolo Davide
Dipartimento di Scienze Chimiche Alimentari Farmaceutiche e Farmacologiche, Università del Piemonte Orientale, Novara I-28100, Italy.
Brain Res. 2003 Nov 21;991(1-2):262-6. doi: 10.1016/j.brainres.2003.08.012.
Embryonic chick ciliary ganglion (CG) neurons obtained from E7-E8 ganglia maintained in serum-free medium were stimulated with 50 microM muscarine. A fast hyperpolarization of the membrane potential was observed in 25% of the cells tested, that in some cases was associated with a slower depolarization. Accordingly, in voltage clamp experiments, either an outward current or a biphasic current response could be observed. Single-channel experiments provide evidence that these signals can be associated to the activation of a K(+) channel whose conductance is 20 pS.
从E7 - E8神经节获取并培养于无血清培养基中的胚胎鸡睫状神经节（CG）神经元，用50微摩尔毒蕈碱刺激。在25%的受试细胞中观察到膜电位快速超极化，在某些情况下这与较慢的去极化相关。因此，在电压钳实验中，可观察到外向电流或双相电流响应。单通道实验提供的证据表明，这些信号可能与电导为20皮安的钾离子通道的激活有关。
