Haasnoot P C Joost, Cupac Daniel, Berkhout Ben
Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
J Biomed Sci. 2003;10(6 Pt 1):607-16. doi: 10.1159/000073526.
RNA interference (RNAi) is a sequence-specific gene-silencing mechanism in eukaryotes, which is believed to function as a defence against viruses and transposons. Since its discovery, RNAi has been developed into a widely used technique for generating genetic knock-outs and for studying gene function by reverse genetics. Additionally, inhibition of virus replication by means of induced RNAi has now been reported for numerous viruses, including several important human pathogens such as human immunodeficiency virus type 1, hepatitis C virus, hepatitis B virus, dengue virus, poliovirus and influenza virus A. In this review, we will summarize the current data on RNAi-mediated inhibition of virus replication and discuss the possibilities for the development of RNAi-based antiviral therapeutics.
RNA干扰(RNAi)是真核生物中一种序列特异性的基因沉默机制,据信它作为一种抵御病毒和转座子的防御机制发挥作用。自发现以来,RNAi已发展成为一种广泛应用的技术,用于产生基因敲除和通过反向遗传学研究基因功能。此外,现已报道通过诱导RNAi抑制多种病毒的复制,包括几种重要的人类病原体,如1型人类免疫缺陷病毒、丙型肝炎病毒、乙型肝炎病毒、登革热病毒、脊髓灰质炎病毒和甲型流感病毒。在本综述中,我们将总结关于RNAi介导的病毒复制抑制的当前数据,并讨论基于RNAi的抗病毒治疗方法的发展可能性。