Park Jung Wan, Jeon Jaemin, Kim Yoosik, Jeon Min Hyok
Department of Internal Medicine, Division of Infectious Disease, Soonchunhyang University Hospital, Cheonan 31151, Republic of Korea.
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
J Clin Med. 2024 Dec 28;14(1):105. doi: 10.3390/jcm14010105.
: This study explores the potential of using elevated levels of blood double-stranded RNA (dsRNA) as a diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) infection. : Blood samples from SFTS patients were collected, dsRNA was purified, and total dsRNA expression was quantitatively analyzed using a spiropyran-based method. Comparative analysis was performed using blood samples from healthy individuals and scrub typhus patients with similar symptoms. : The results revealed that individuals infected with SFTS had significantly higher total blood dsRNA levels compared to healthy or scrub typhus controls. The dsRNA-based method also has potential for assessing infection severity based on dsRNA levels. : These findings suggest that total dsRNA expression can serve as a quick and convenient method to differentiate SFTS from other non-viral conditions with similar clinical presentations. This method shows promise as a novel diagnostic tool.
本研究探讨了将血液双链RNA（dsRNA）水平升高用作严重发热伴血小板减少综合征（SFTS）感染诊断工具的潜力。收集了SFTS患者的血样，纯化了dsRNA，并使用基于螺吡喃的方法对总dsRNA表达进行了定量分析。使用来自健康个体和具有相似症状的恙虫病患者的血样进行了比较分析。结果显示，与健康对照组或恙虫病对照组相比，感染SFTS的个体血液中总dsRNA水平显著更高。基于dsRNA的方法也具有根据dsRNA水平评估感染严重程度的潜力。这些发现表明，总dsRNA表达可作为一种快速便捷的方法，用于区分SFTS与其他具有相似临床表现的非病毒疾病。该方法有望成为一种新型诊断工具。
