Artiukhov V G, Basharina O V, Iskusnykh A Iu
Voronezh State University, Russia.
Ukr Biokhim Zh (1999). 2003 May-Jun;75(3):45-9.
The dynamics of structural and functional changes proceeding in a peroxidase molecule under the effect of temperature was studied. It was shown that peroxidase thermoinactivation proceeds in two consequent stages. Based on the analysis of enzyme peroxidase and oxidase activity and peroxidase spectral and buffer characteristics, it was established that at temperatures from 20 to 55 degrees C reversible conformation there occur changes of the hemoprotein molecule related to consequent unfolding and folding of the protein globule. The influence of temperature of 60 degrees C and above induces the protein globule unfolding and loosing of peroxidase activity.
研究了温度作用下过氧化物酶分子中发生的结构和功能变化动态。结果表明，过氧化物酶热失活分两个连续阶段进行。基于对酶的过氧化物酶和氧化酶活性以及过氧化物酶光谱和缓冲特性的分析，确定在20至55摄氏度的温度下，血红蛋白分子会发生可逆构象变化，这与蛋白质球状体的相继展开和折叠有关。60摄氏度及以上温度的影响会导致蛋白质球状体展开并丧失过氧化物酶活性。
