Kaliman P A, Inshina N N, Strel'chenko E V
Karazin Kharkov National University, Ukraine.
Ukr Biokhim Zh (1999). 2003 May-Jun;75(3):109-14.
The 5-aminolevulinate synthase, heme oxygenase, tryptophan-2,3-dioxygenase activities, the content of total heme and cytochrome P-450 content in the rat liver and absorption spectrum of blood serum in Soret region under glycerol model of rhabdomiolisis and hemolytic anemia caused by single phenylhydrazine injection have been investigated. The glycerol injection caused a considerable accumulation of heme-containing products in the serum and the increase of the total heme content, holoenzyme, total activity and heme saturation of tryptophan-2,3-dioxygenase, as well as the increase of the 5-aminolevulinate synthase and heme oxygenase activities in the liver during the first hours of its action and the decrease of cytochrome P-450 content in 24 h. Administration of phenylhydrazine lead to the increasing of hemolysis products content in blood serum too, although it was less expressed. The phenylhydrazine injection caused the increase of activities of 5-aminolevulinate synthase, holoenzyme, total activity and heme saturation of tryptophan-2,3-dioxygenase, as well as decrease of cytochrome P-450 content in the rat liver in 2 h. The increase of the total heme content and heme oxygenase activity has been observed in 24 h. The effect of heme arrival from the blood stream, as well as a direct influence of glycerol and phenylhydrazine on the investigated parameters are discussed.
研究了在甘油诱导的横纹肌溶解和单次注射苯肼引起的溶血性贫血模型中，大鼠肝脏中5-氨基酮戊酸合酶、血红素加氧酶、色氨酸-2,3-双加氧酶的活性、总血红素含量、细胞色素P-450含量以及血清在Soret区的吸收光谱。注射甘油导致血清中含血红素产物大量积累，总血红素含量增加，色氨酸-2,3-双加氧酶的全酶、总活性和血红素饱和度增加，以及在其作用的最初几小时内肝脏中5-氨基酮戊酸合酶和血红素加氧酶活性增加，24小时后细胞色素P-450含量降低。注射苯肼也导致血清中溶血产物含量增加，尽管程度较轻。注射苯肼导致大鼠肝脏中5-氨基酮戊酸合酶、色氨酸-2,3-双加氧酶的全酶、总活性和血红素饱和度增加，以及2小时后细胞色素P-450含量降低。24小时后观察到总血红素含量和血红素加氧酶活性增加。讨论了来自血流的血红素的影响，以及甘油和苯肼对所研究参数的直接影响。
