Zhou Yu-Chuan, Fu Qi-Gao, Zhao Ru-Qian, Ni Ying-Dong, Chen Jie
Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2003 Sep;30(9):840-6.
Expression of genes encoding growth hormone receptor (GHR), type I insulin-like growth factor receptor (IGF-IR), follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) was measured in granulosa and theca layers of the largest (F1), third largest (F3), fifth largest (F5) preovulatory follicles and large white follicles (LWF) in the ovary of Shaoxing ducks, with relative quantitative reverse transcription-polymerase chain reaction (RT-PCR) using beta-actin as an internal standard. The results showed that GHR mRNA was more abundant in theca layer than in granulosa layer in all the follicles investigated, in theca layer, the LWF follicle expressed highest the level of GHR mRNA, while no differences in granulosa layer were observed among follicles at different stages of development. In contrast, the expression of IGF-IR mRNA in theca layer was evidently lower than that in granulosa layer, but no significant changes were found among different stages of follicles in either layers, except that LWF trended to express higher IGF-IR mRNA in the theca layer compared to other preovulatory follicles. Similar to IGF-IR mRNA, FSHR mRNA was more abundant in granulosa layer and no significant differences were found among different stages of follicles in either layers. In contrast, the expression of LHR mRNA followed a different pattern. In the theca layer, the expression level maintained consistent, while in the granulosa layer, a significant stepwise increase was observed as the follicles matured, resulting in higher mRNA level of LHR in the granulosa layer of largest follicles (F1), but lower mRNA level of LHR in the granulosa layer of smaller follicles (F5 and LWF). These results suggest that GH and IGF-I regulate ovary function by respective dominant sites in the follicles, and they cooperate with LH and FSH to regulate the follicle development via their respective receptors. The high expression of LHR mRNA in granulosa layer of large follicles might be related to the establishment of the follicle hierarchy and ovulation.
以β-肌动蛋白为内参，采用相对定量逆转录聚合酶链反应（RT-PCR）技术，检测绍兴鸭卵巢中最大（F1）、第三大（F3）、第五大（F5）排卵前卵泡及大白卵泡（LWF）的颗粒层和卵泡膜层中生长激素受体（GHR）、Ⅰ型胰岛素样生长因子受体（IGF-IR）、促卵泡激素受体（FSHR）和促黄体生成素受体（LHR）编码基因的表达。结果显示，在所研究的所有卵泡中，卵泡膜层的GHR mRNA含量均高于颗粒层；在卵泡膜层中，LWF卵泡的GHR mRNA表达水平最高，而在颗粒层中，不同发育阶段的卵泡之间未观察到差异。相反，卵泡膜层中IGF-IR mRNA的表达明显低于颗粒层，且在两层的不同卵泡阶段均未发现显著变化，只是与其他排卵前卵泡相比，LWF卵泡膜层中的IGF-IR mRNA表达有升高趋势。与IGF-IR mRNA相似，FSHR mRNA在颗粒层中的含量更高，且在两层的不同卵泡阶段均未发现显著差异。相比之下，LHR mRNA的表达模式不同。在卵泡膜层中，其表达水平保持一致；而在颗粒层中，随着卵泡成熟，观察到显著的逐步增加，导致最大卵泡（F1）颗粒层中的LHR mRNA水平较高，而较小卵泡（F5和LWF）颗粒层中的LHR mRNA水平较低。这些结果表明，GH和IGF-I通过卵泡中的各自优势位点调节卵巢功能，并且它们通过各自的受体与LH和FSH协同调节卵泡发育。大卵泡颗粒层中LHR mRNA的高表达可能与卵泡等级的建立和排卵有关。
