Nakayama Y, Matsuda T
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
ASAIO J. 1992 Jul-Sep;38(3):M421-4.
The authors report a new photochemical technique for the fixation of hydrogels onto the surface of fabricated devices and sensors. The preparative method is based on two different types of photochemistry: photocross-linking due to intermolecular photodimerization of cinnamoyl groups, and photoinduced covalent bonding due to the photoreactivity of azido groups. The immobilization of heparin, useful as a thromboresistant coating, onto poly(ethylene terephthalate), and the immobilization of glucose oxidase onto the surface of a carbon fiber electrode, were demonstrated. In the former, immobilized heparin was found to be continuously released from the hydrogel, and the release rate was effectively controlled by the thickness of the hydrogel. In the latter, upon the addition of glucose, a spontaneous electrical response was observed to function as a glucose sensor. No delamination was found for either system after vigorous washing with water. Thus, durable surface hydrogelation, simultaneous immobilization of bioactive substances, and functioning of the systems were demonstrated.
作者报告了一种用于将水凝胶固定在制造的设备和传感器表面的新光化学技术。该制备方法基于两种不同类型的光化学：由于肉桂酰基团的分子间光二聚作用引起的光交联，以及由于叠氮基团的光反应性引起的光诱导共价键合。证明了作为抗血栓涂层有用的肝素固定在聚对苯二甲酸乙二酯上，以及葡萄糖氧化酶固定在碳纤维电极表面。在前者中，发现固定的肝素从水凝胶中持续释放，并且释放速率可通过水凝胶的厚度有效控制。在后者中，加入葡萄糖后，观察到自发的电响应，其作为葡萄糖传感器起作用。用水剧烈洗涤后，两个系统均未发现分层现象。因此，证明了持久的表面水凝胶化、生物活性物质的同时固定以及系统的功能。
