与BRCA2相互作用的蛋白质DSS1对玉米黑粉菌中的DNA修复、重组和基因组稳定性至关重要。

The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydis.

作者信息

Kojic Milorad, Yang Haijuan, Kostrub Corwin F, Pavletich Nikola P, Holloman William K

机构信息

Department of Microbiology and Immunology, Hearst Microbiology Research Center, Weill Medical College of Cornell University, New York, NY 10021, USA.

出版信息

Mol Cell. 2003 Oct;12(4):1043-9. doi: 10.1016/s1097-2765(03)00367-8.

DOI:10.1016/s1097-2765(03)00367-8

PMID:14580353

Abstract

DSS1 encodes a small acidic protein shown in recent structural studies to interact with the DNA binding domain of BRCA2. Here we report that an ortholog of DSS1 is present in Ustilago maydis and associates with Brh2, the BRCA2-related protein, thus recapitulating the protein partnership in this genetically amenable fungus. Mutants of U. maydis deleted of DSS1 are extremely radiation sensitive, deficient in recombination, defective in meiosis, and disturbed in genome stability; these phenotypes mirror previous observations of U. maydis mutants deficient in Brh2 or Rad51. These findings conclusively show that Dss1 constitutes a protein with a significant role in the recombinational repair pathway in U. maydis, and imply that it plays a similar key role in the recombination systems of organisms in which recombinational repair is BRCA2 dependent.

摘要

DSS1编码一种小的酸性蛋白，近期的结构研究表明该蛋白可与BRCA2的DNA结合结构域相互作用。在此我们报告，玉米黑粉菌中存在DSS1的一个直系同源物，它与BRCA2相关蛋白Brh2相关联，从而在这种易于进行基因操作的真菌中重现了这种蛋白伙伴关系。缺失DSS1的玉米黑粉菌突变体对辐射极其敏感，重组缺陷，减数分裂有缺陷，基因组稳定性受到干扰；这些表型与之前观察到的缺乏Brh2或Rad51的玉米黑粉菌突变体一致。这些发现确凿地表明，Dss1在玉米黑粉菌的重组修复途径中构成一种具有重要作用的蛋白，这意味着它在重组修复依赖BRCA2的生物体的重组系统中发挥类似的关键作用。