Lucotte G, Champenois T
Centre de Neurogénétique Moléculaire, 44 rue Monge, 75005 Paris, France.
Mol Cell Probes. 2003 Oct;17(5):267-9. doi: 10.1016/j.mcp.2003.07.008.
The authors have used a primer-engineered duplex polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) for the simultaneous detection of factor V Leiden and prothrombin G20210A mutations. The method involves the generation of HindIII RFLPs, and the restricted amplification products are analyzed by agarose gel electrophoresis in a single gel lane. This method is simple an inexpensive, and readily adaptable to the routine in a clinical molecular diagnostic laboratory other that our own.
作者采用了引物工程化双链聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）方法同时检测凝血因子V Leiden突变和凝血酶原G20210A突变。该方法涉及产生HindIII RFLP，限制性扩增产物在单一凝胶泳道中通过琼脂糖凝胶电泳进行分析。此方法简单、廉价，并且除了我们自己的实验室外，很容易适用于其他临床分子诊断实验室的常规检测。
