Young Joshua K, Orth Anthony P, Goodman Walter G
Department of Entomology, University of Wisconsin-Madison, 237 Russell Laboratory, 1630 Linden Dr., Madison, WI 53706, USA.
Mol Cell Endocrinol. 2003 Oct 31;208(1-2):41-50. doi: 10.1016/s0303-7207(03)00259-4.
Peripheral distribution of the insect juvenile hormones (JHs) requires hemolymph transport proteins. A comparison of three strains of the tobacco hornworm Manduca sexta indicates that hemolymph JH binding protein (hJHBP) levels in the Madison wild-type (Mwt) strain are significantly higher than in the black larval mutant (bl) and Seattle wild-type (Swt) strains. To correlate differences in hJHBP levels between strain phenotypes with the hJHBP locus, we sequenced 8.4 kb of the hJHBP gene locus from each strain. Snb, an allele found in the Swt and bl strains, contains a 408 bp repetitive nuclear element flanked by 15 bp direct repeats. Mating studies coupled with molecular genotyping demonstrate that the presence of Snb correlates with a twofold lower hJHBP level relative to the allele found in the Mwt strain. Despite the lower hJHBP levels in individuals carrying the Snb gene, hemolymph levels of JH do not appear to be significantly affected.
昆虫保幼激素（JH）的外周分布需要血淋巴转运蛋白。对烟草天蛾曼陀罗六代的三个品系进行比较表明，麦迪逊野生型（Mwt）品系中的血淋巴JH结合蛋白（hJHBP）水平显著高于黑色幼虫突变体（bl）和西雅图野生型（Swt）品系。为了将品系表型之间hJHBP水平的差异与hJHBP基因座相关联，我们对每个品系的hJHBP基因座的8.4 kb进行了测序。Snb是在Swt和bl品系中发现的一个等位基因，包含一个408 bp的重复核元件，两侧有15 bp的直接重复序列。交配研究与分子基因分型表明，Snb的存在与相对于Mwt品系中发现的等位基因hJHBP水平降低两倍相关。尽管携带Snb基因的个体中hJHBP水平较低，但JH的血淋巴水平似乎没有受到显著影响。
