Scholz Stefan, Kordes Claus, Hamann Juliane, Gutzeit Herwig O
Institute of Zoology, University of Dresden, D-01062, Germany.
Mar Environ Res. 2004 Apr;57(3):235-44. doi: 10.1016/S0141-1136(03)00082-5.
Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.
鱼类卵黄前体黄前体卵黄蛋白原（VTG）的定量分析已成为检测已知化学物质和环境样品雌激素效应的标准技术。在本研究中，我们分析了在模式硬骨鱼青鳉（Oryzias latipes）中，雌二醇、乙炔雌二醇和染料木黄酮暴露对VTG的诱导作用，并证明青鳉是分析雌激素效应的合适模型系统。通过比较VTG基因表达和蛋白质水平，我们表明，原则上这两种技术均可用于研究体内（幼年和成年雄性）和体外（雄性肝细胞原代培养物）的VTG诱导。如果进行短期体内或体外暴露，检测mRNA可能就足够了。对于需要检测弱雌激素化学物质并进行精确定量的长期研究，免疫化学检测可能更受青睐。
