Kondo Eiji, Yasuda Kazunori, Yamanaka Masanori, Minami Akio, Tohyama Harukazu
Department of Medical Bioengineering and Sports Medicine, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan.
Clin Biomech (Bristol). 2003 Dec;18(10):942-9. doi: 10.1016/s0268-0033(03)00171-2.
To biomechanically evaluate a newly devised model for the elongation-type anterior cruciate ligament injury with partial laceration and permanent elongation.
Thirty-six rabbits were randomly divided into 4 groups of 9 animals each, after a quantitative injury was given to the right anterior cruciate ligament. The 4 groups were sacrificed at 0, 6, 12, and 24 weeks after surgery, respectively. Biomechanical and histological evaluations were performed at each period.
No adequate animal models have been established for the elongation-type anterior cruciate ligament injury in which the mid-substance is permanently elongated with partial laceration.
The anteromedial and posterolateral half of the anterior cruciate ligament was transected at the proximal and distal one-third levels, respectively. Then, the anterior cruciate ligament was stretched by applying an anterior drawer force to the tibia at 90 degrees of knee flexion.
The treatment significantly increased the anterior translation of the knee into approximately 150-250% at each period after surgery. The maximum load and the stiffness of the femur-anterior cruciate ligament-tibia complex significantly decreased to 30% or less immediately after surgery, and then gradually increased to 50% at 12 weeks.
In this model, this quantitative treatment created serious injuries with partial laceration and permanent elongation in the anterior cruciate ligament to similar degrees. Also, incomplete tissue healing occurred in the anterior cruciate ligament to similar degrees after the treatment.
This model will be useful to study new therapeutic methods for the elongation-type anterior cruciate ligament injury.
对一种新设计的用于模拟部分撕裂并伴有永久性延长的伸长型前交叉韧带损伤的模型进行生物力学评估。
对三十六只兔子的右前交叉韧带进行定量损伤后，将其随机分为四组，每组九只动物。四组分别在术后0、6、12和24周处死。在每个时间段进行生物力学和组织学评估。
对于中份实质永久性延长且伴有部分撕裂的伸长型前交叉韧带损伤，尚未建立足够的动物模型。
分别在近端和远端三分之一水平横断前交叉韧带的前内侧和后外侧半部分。然后，在膝关节屈曲90度时对胫骨施加前抽屉力来拉伸前交叉韧带。
该治疗在术后各时间段均显著增加了膝关节的前向平移，幅度约为150%至250%。股骨-前交叉韧带-胫骨复合体的最大负荷和刚度在术后立即显著降低至30%或更低，然后在12周时逐渐增加至50%。
在该模型中，这种定量治疗在前交叉韧带中造成了类似程度的严重部分撕裂和永久性延长损伤。此外，治疗后前交叉韧带中也出现了类似程度的不完全组织愈合。
该模型将有助于研究伸长型前交叉韧带损伤的新治疗方法。
