二维毛细管电泳，包括毛细管等电聚焦和毛细管区带电泳。

Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis.

作者信息

Yang Chun, Zhang Lingyi, Liu Hechun, Zhang Weibing, Zhang Yukui

机构信息

National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhong Shan Road, Dalian 116011, PR China.

出版信息

J Chromatogr A. 2003 Nov 7;1018(1):97-103. doi: 10.1016/j.chroma.2003.08.031.

DOI:10.1016/j.chroma.2003.08.031

PMID:14582630

Abstract

Capillary isoelectric focusing (cIEF) and capillary zone electrophoresis (CZE) was on-line hyphenated by a dialysis interface to achieve a 2D capillary electrophoresis (CE) system. The system was used with just one high-voltage power supply and three electrodes (one cathode shared by the two dimensions). The focused zone in the first dimension (i.e. the cIEF) was driven to the dialysis interface by electroosmotic flow (EOF), besides chemical mobilization from the first anode to the shared cathode. And then in the second dimension (i.e. the CZE), the separated zone was further separated and driven by an inverted EOF, which originated from the charged layer of a cationic surfactant adsorbed onto the inner wall of the capillary. Finally, a solution of ribonuclease was rapidly separated to assess the feasibility of the two-dimensional CE implement.

摘要

通过透析接口将毛细管等电聚焦（cIEF）和毛细管区带电泳（CZE）在线联用，以实现二维毛细管电泳（CE）系统。该系统仅使用一个高压电源和三个电极（两个维度共用一个阴极）。在第一维（即cIEF）中，聚焦区除了从第一个阳极向共用阴极进行化学迁移外，还通过电渗流（EOF）驱动至透析接口。然后在第二维（即CZE）中，分离区由源自吸附在毛细管内壁的阳离子表面活性剂带电层的反向EOF进一步分离并驱动。最后，对核糖核酸酶溶液进行快速分离，以评估二维CE仪器的可行性。