Pergande Melissa R, Cologna Stephanie M
Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA.
Proteomes. 2017 Jan 25;5(1):4. doi: 10.3390/proteomes5010004.
The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies.
根据等电点分离两性成分在肽和蛋白质的分离、降低复杂性及提高检测方面发挥了重要作用。本简要综述概述了等电聚焦的基本原理，包括历史成就总结及实验设计考量。还讨论了等电聚焦的衍生方法，包括常用的检测方法。介绍了基于等电点分离在各个领域的应用以及该领域未来研究展望。
