Zhang Aijun, Polavarapu Sridhar
USDA, Agriculture Research Services, Chemicals Affecting Insect Behavior Laboratory, Beltsville Agriculture Research Center-West, Beltsville, Maryland 20705, USA.
J Chem Ecol. 2003 Sep;29(9):2153-64. doi: 10.1023/a:1025646804797.
The cranberry blossom worm, Epiglaea apiata (Grote) (Lepidoptera: Noctuidae), is a major pest of cranberries in New Jersey. The female sex pheromone of this moth was identified as a blend of (Z)-9-hexadecenyl acetate (Z9-16:Ac), (Z)-9-tetradecenyl acetate (Z9-14:Ac), and tetradecyl acetate (14:Ac) by gas chromatographic-electroantennographic detection and gas chromatography-mass spectrometry. The ratio of the components in extracts of the female pheromone gland was determined to be 65:2:33 of the Z9-16:Ac, Z9-14:Ac, and 14:Ac, respectively. The double bond positions of the pheromone components were confirmed by dimethyl disulfide derivatization. In addition to the above three components, a mixture of C4-C10 aliphatic acids was present in both gland extracts and effluvia collections, and the acids elicited significant EAD responses from male moth antennae. However, addition of the C4-C10 aliphatic acids to the pheromone blend did not significantly increase trap captures. Three-hundred- and 1000-microg doses of a synthetic blend containing Z9-16:Ac, Z9-14:Ac, and 14:Ac (65:2:33), on a rubber septum were more attractive to males than lower doses.
蔓越橘花虫,Epiglaea apiata(格罗特)(鳞翅目:夜蛾科),是新泽西州蔓越橘的主要害虫。通过气相色谱 - 触角电位检测和气相色谱 - 质谱联用技术,鉴定出这种蛾的雌性性信息素是由(Z)-9-十六碳烯基乙酸酯(Z9-16:Ac)、(Z)-9-十四碳烯基乙酸酯(Z9-14:Ac)和十四烷基乙酸酯(14:Ac)混合而成。雌性性信息素腺体提取物中各成分的比例分别确定为Z9-16:Ac、Z9-14:Ac和14:Ac的65:2:33。通过二甲基二硫醚衍生化确定了性信息素成分的双键位置。除了上述三种成分外,腺体提取物和气味收集物中都存在C4 - C10脂肪酸混合物,这些酸能引起雄蛾触角显著的触角电位反应。然而,在性信息素混合物中添加C4 - C10脂肪酸并没有显著增加诱捕量。在橡胶隔片上含有Z9-16:Ac、Z9-14:Ac和14:Ac(65:2:33)的300微克和1000微克合成混合物剂量对雄蛾比低剂量更具吸引力。