Chen Xiong, Nakamuta Kiyoshi, Nakanishi Tomoaki, Nakashima Tadakazu, Tokoro Masahiko, Mochizuki Fumiaki, Fukumoto Takehiko
Department of Forest Entomology, Forestry and Forest Products Research Institute, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki, Japan.
J Chem Ecol. 2006 Mar;32(3):669-79. doi: 10.1007/s10886-005-9025-4. Epub 2006 Apr 26.
This study describes the identification of a sex pheromone component of a cossid moth, Cossus insularis. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analysis of solid-phase microextraction (SPME) collections of volatiles released by live female moths showed that two compounds elicited EAG responses from the antennae of male moths. These compounds were identified as (E)-3-tetradecenyl acetate (E3-14:Ac) and (Z)-3-tetradecenyl acetate (Z3-14:Ac) by mass spectral analysis and retention index comparisons with synthetic standards. The ratio of E3-14:Ac and Z3-14:Ac was 95:5 in the effluvia of a female. In field bioassays, sticky traps baited with blends of E3-14:Ac and Z3-14:Ac showed that E3-14:Ac is an essential component of the pheromone. However, the role of Z3-14:Ac is unclear, because E3-14:Ac as a single component was as attractive to male moths as blends of E3-14:Ac and Z3-14:Ac, including the 95:5 blend released by live female moths.
本研究描述了一种木蠹蛾——岛屿木蠹蛾性信息素成分的鉴定过程。对活体雌蛾释放的挥发性物质进行固相微萃取(SPME)收集,并通过气相色谱 - 触角电位检测联用(GC - EAD)分析,结果显示有两种化合物能引起雄蛾触角的触角电位反应。通过质谱分析以及与合成标准品的保留指数比较,这些化合物被鉴定为(E)-3 - 十四碳烯乙酸酯(E3 - 14:Ac)和(Z)-3 - 十四碳烯乙酸酯(Z3 - 14:Ac)。雌蛾释放物中E3 - 14:Ac与Z3 - 14:Ac的比例为95:5。在田间生物测定中,用E3 - 14:Ac和Z3 - 14:Ac混合物诱捕的粘性诱捕器表明,E3 - 14:Ac是性信息素的重要成分。然而,Z3 - 14:Ac的作用尚不清楚,因为E3 - 14:Ac作为单一成分对雄蛾的吸引力与E3 - 14:Ac和Z3 - 14:Ac的混合物相当,包括活体雌蛾释放的95:5的混合物。