Suh Kahp Y, Seong Jiehyun, Khademhosseini Ali, Laibinis Paul E, Langer Robert
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Biomaterials. 2004 Feb;25(3):557-63. doi: 10.1016/s0142-9612(03)00543-x.
We present a simple, direct soft lithographic method to fabricate poly(ethylene glycol) (PEG) microstructures for protein and cell patterning. This lithographic method involves a molding process in which a uniform PEG film is molded with a patterned polydimethylsiloxane stamp by means of capillary force. The patterned surfaces created by this method provide excellent resistance towards non-specific protein and cell adsorption. The patterned substrates consist of two regions: the molded PEG surface that acts as a resistant layer and the exposed substrate surface that promotes protein or cell adsorption. A notable finding here is that the substrate surface can be directly exposed during the molding process due to the ability to control the wetting properties of the polymer on the stamp, which is a key factor to patterning proteins and cells.
我们展示了一种简单、直接的软光刻方法，用于制造用于蛋白质和细胞图案化的聚乙二醇（PEG）微结构。这种光刻方法涉及一个模制过程，其中通过毛细作用力用图案化的聚二甲基硅氧烷印章对均匀的PEG膜进行模制。通过这种方法创建的图案化表面对非特异性蛋白质和细胞吸附具有出色的抗性。图案化的基底由两个区域组成：作为抗性层的模制PEG表面和促进蛋白质或细胞吸附的暴露基底表面。这里一个值得注意的发现是，由于能够控制聚合物在印章上的润湿性，基底表面在模制过程中可以直接暴露，这是对蛋白质和细胞进行图案化的关键因素。
